PurePrep Body Fluid Kit

The PurePrep Body Fluid Kit allows fast and cost-effective extraction of genomic DNA from blood and saliva samples. The magnetic bead-based kit can be used on fresh or frozen whole blood and fresh or preserved saliva samples. The ready-to-use reagents and simple protocol are convenient in use and easy to automate. As a linear volume to volume ratio is used between sample and reagents, it is possible to use the kit in any situation where high quality genomic DNA is needed.

Bead Beater
Features
Contents
Specifications
MolGen - Pippet

Fast and efficient purification of circulating cell-free DNA from human plasma or serum samples

  • Consistently high yields of nucleic acids
  • Easy to use protocols (~60 minutes for 24 samples)
  • Optimized for use on PurePrep 24
  • No additional alcohols required
  • After lysis at 56°C all other steps are done at room temperature
  • Typical yield: 0.5 to 4 ng cfDNA per mL of human plasma (highly variable from donor to donor)
  • Well suited for many enzymatic down-stream applications in particular RT-qPCR and sequencing
MolGen - Pippet

Contents

  • Lysis Buffer U1
  • Binding Buffer U1
  • Proteinase K
  • Wash Buffer I
  • Wash Buffer II
  • Elution Buffer
  • MagSi-BF 9

Specifications

Input volume 1 to 4 mL sample
Output Volume 60 µL
Processing time for 96 samples ~60 min
Sample Types Fresh or frozen plasma or serum samples
Processing Method Manual or automated
Marking Research Use Only

 

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