PurePrep cfDNA Kit

The PurePrep cfDNA kit allows (automated) purification of circulating cell-free DNA from human plasma or serum samples. Processing time for the automated preparation of 24 samples is about 60 minutes. The kit requires no phenol/chloroform extraction or alcohol precipitation and eliminates the need for repeated centrifugation, vacuum filtration or column separation. It allows safe handling of potentially infectious samples. The obtained cfDNA can be used directly for downstream applications such as qPCR, or any kind of enzymatic reaction.

Bead Beater
MolGen - Pippet

Fast and efficient purification of circulating cell-free DNA from human plasma or serum samples

  • Consistently high yields of nucleic acids
  • Easy to use protocols (~60 minutes for 24 samples)
  • Optimized for use on PurePrep 24
  • No additional alcohols required
  • After lysis at 56°C all other steps are done at room temperature
  • Typical yield: 0.5 to 4 ng cfDNA per mL of human plasma (highly variable from donor to donor)
  • Well suited for many enzymatic down-stream applications in particular RT-qPCR and sequencing
PurePrep 24 Inside


  • Lysis Buffer CF
  • Binding Buffer CF
  • Proteinase K
  • MagSi-CF8
  • Elution Buffer
  • Wash Buffer CF1
  • Wash Buffer CF2


Input volume 1 to 4 mL sample
Output Volume 60 µL
Processing time for 96 samples ~60 min
Sample Types Fresh or frozen plasma or serum samples
Processing Method Manual or automated
Marking Research Use Only


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