PurePrep Tissue & Cells Kit
Bead-based purification kit for tissue samples and eukaryotic cells
Fast and efficient purification of circulating cell-free DNA
The PurePrep cfDNA Kit comes complete with all necessary chemicals for the extraction process of circulating cell-free DNA (no additional alcohols required)
The kit requires no phenol/chloroform extraction or alcohol precipitation and eliminates the need for repeated centrifugation, vacuum filtration or column separation. Allowing safe handling of potentially infectious samples.
The obtained cfDNA can be used directly for downstream applications such as qPCR, or any kind of enzymatic reaction.
The kit requires no phenol/chloroform extraction or alcohol precipitation and eliminates the need for repeated centrifugation, vacuum filtration or column separation. Allowing safe handling of potentially infectious samples.
The obtained cfDNA can be used directly for downstream applications such as qPCR, or any kind of enzymatic reaction.
Features
- Consistently high yields of nucleic acids
- Easy to use protocols (~60 minutes for 24 samples)
- Optimized for use on PurePrep 24
- No additional alcohols required
- After lysis at 56°C all other steps are done at room temperature
- Typical yield: 0.5 to 4 ng cfDNA per mL of human plasma (highly variable from donor to donor)
- Well suited for many enzymatic down-stream applications in particular RT-qPCR and sequencing
Specifications
Contents:
- Lysis Buffer CF
- Binding Buffer CF
- Proteinase K
- MagSi-CF8
- Elution Buffer
- Wash Buffer CF1
- Wash Buffer CF2
Available in:
- 96 Preps
