PurePrep Tissue & Cells Kit

The PurePrep Tissue & Cells kit allows manual and automated isolation of genomic DNA from mammalian tissue samples and eukaryotic cells. Processing time for the preparation of 96 samples is about 40 minutes plus an additional pre-lysis incubation of 15 min (for cells), 1-3 h or overnight for tissue samples. The kit does not require phenol/chloroform extraction or alcohol precipitation and eliminates the need for repeated centrifugation, vacuum filtration or column separation. It allows safe handling of potentially infectious samples, and is designed to avoid sample-to-sample cross-contamination. The obtained DNA can be used directly for downstream applications such as PCR or any kind of enzymatic reaction.

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Features

Contents

Specifications

Fast and efficient purification of circulating cell-free DNA from human plasma or serum samples

Consistently high yields of nucleic acids
Easy to use protocols (~60 minutes for 24 samples)
Optimized for use on PurePrep 24
No additional alcohols required
After lysis at 56°C all other steps are done at room temperature
Typical yield: 0.5 to 4 ng cfDNA per mL of human plasma (highly variable from donor to donor)
Well suited for many enzymatic down-stream applications in particular RT-qPCR and sequencing

Lysis Buffer TS
Binding Buffer U1
Wash Buffer I
Wash Buffer II
Elution Buffer
Proteinase K
MagSi-TC VI

Specifications

Input volume	1 to 4 mL sample
Output Volume	60 µL
Processing time for 96 samples	~60 min
Sample Types	Fresh or frozen plasma or serum samples
Processing Method	Manual or automated
Marking	Research Use Only