PurePrep Tissue & Cells Kit

The PurePrep Tissue & Cells kit allows manual and automated isolation of genomic DNA from mammalian tissue samples and eukaryotic cells. Processing time for the preparation of 96 samples is about 40 minutes plus an additional pre-lysis incubation of 15 min (for cells), 1-3 h or overnight for tissue samples. The kit does not require phenol/chloroform extraction or alcohol precipitation and eliminates the need for repeated centrifugation, vacuum filtration or column separation. It allows safe handling of potentially infectious samples, and is designed to avoid sample-to-sample cross-contamination. The obtained DNA can be used directly for downstream applications such as PCR or any kind of enzymatic reaction.

Bead Beater
MolGen - Pippet

Fast and efficient purification of circulating cell-free DNA from human plasma or serum samples

  • Consistently high yields of nucleic acids
  • Easy to use protocols (~60 minutes for 24 samples)
  • Optimized for use on PurePrep 24
  • No additional alcohols required
  • After lysis at 56°C all other steps are done at room temperature
  • Typical yield: 0.5 to 4 ng cfDNA per mL of human plasma (highly variable from donor to donor)
  • Well suited for many enzymatic down-stream applications in particular RT-qPCR and sequencing
PurePrep 24 Inside


  • Lysis Buffer TS
  • Binding Buffer U1
  • Wash Buffer I
  • Wash Buffer II
  • Elution Buffer
  • Proteinase K
  • MagSi-TC VI


Input volume 1 to 4 mL sample
Output Volume 60 µL
Processing time for 96 samples ~60 min
Sample Types Fresh or frozen plasma or serum samples
Processing Method Manual or automated
Marking Research Use Only


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