pureprep plant 0.0 kit

Introducing the PurePrep Plant 0.0 kit – Alcohol-Free extraction reagents

PATENT PENDING

The PurePrep Plant 0.0 Kit from MolGen is designed for ultra-fast DNA extraction from a variety of plant material, with an automated extraction run taking as little as 10 minutes.

The kit is supplied with a single lysis buffer compatible with both leaf and seed material, with all reagents completely free of alcoholic components, and harsh chaotropic salts. The absence of alcoholic components means that no bead drying time is necessary, resulting in rapid automated processing from lysate to eluate.

MolGen is helping you move towards a safer lab environment, with the absence of alcoholic components ensuring reagents are safer to transport, use, and store. To move you closer to your CSR / ESG goals, the absence of alcohol
and chaotropic components in the PurePrep Plant 0.0 kit makes waste much easier to dispose of in an environmentally friendly manner.

The extraction process is optimally automated on the MolGen PurePrep extraction family, the PurePrep GenePrep System and DNA Factory, as well as other automated systems compatible with bead-based chemistries. The quality and quantity of the extracted DNA is excellent, and suitable to be used in a wide variety of applications.

DNA Yield

Species n Average DNA yield
(ng/µl)
Seed Wheat​ 14​ 8.6​
Corn​ 14​ 21.2​
Rapeseed​ 8​ 5.7​
Sunflower​ 8​ 44.9​
Leaf Spinach​ 8​ 4.4​
Lettuce​ 8​ 1.5​
Leek​ 8​ 8.7​
Parsley​ 8​ 6.6​

18S Plant PCR – PurePrep Plant 0.0

Species n Ct Value
Seed Wheat​ 4 18.98
Corn​ 8 19,29
Rapeseed​ 4 14.87
Sunflower​ 4 15.86
Leaf Spinach​ 4 17.65
Lettuce​ 4 19.95
Leek​ 4 19.66
Parsley​ 4 18.75

Corn samples 5x diluted, all other samples undiluted

Comparison Data PurePrep Plant Kit vs PurePrep Plant 0.0 Kit

Comparison data from 8 different species extracted with PurePrep Universal Plant Kit (ethanol free) vs PurePrep Plant 0.0 (fully alcohol free)

MolGen is a global total solution provider of innovative extraction DNA/RNA technology, systems, products and kits for human and animal diagnostics, the agriculture and biotech industries. MolGen’s, systems, reagents and consumables reduce hands-on time and increase productivity while maintaining high yields and reproducibility. From sample to result, MolGen is challenging the status quo in molecular diagnostics by offering platform stand-alone systems and consumables. MolGen’s portfolio offers high-quality, adaptable, and safe workflows, which can be customised to meet customers’ specific needs.