Bead-based reagent kit to extract DNA from leaf and seed samples
The PurePrep Universal Plant Kit from MolGen is designed for ultra-fast DNA extraction from 20-50mg of leaf or seed samples, with a standard run on a PurePrep 96 instrument taking approximately 10 minutes.
The kit uses a single lysis buffer which is compatible with both leaf and seed materials and includes a precipitation buffer to facilitate optimal cleared lysate transfer.
The purification process is easily automatable on the PurePrep Extraction Systems, the GenePrep System as well as other automated systems compatible with bead-based chemistries.
The kit contains no alcohol-based washes with only isopropanol present in the binding buffer, leading to an overal reduction of liquid alcoholic waste. There is also no drying time due to the absence of ethanol in washes, allowing for greatly reduced processing time.
The precipitation buffer allows for better pelleting of cellular debris prior to lysate transfer.
The quality and quantity of the extracted DNA is excellent and allows for the DNA to be used in a wide variety of applications.
Features
Contains single lysis buffers for both plant and leaf sample types
Standard run on PurePrep 96: ~10 min
High yields and excellent DNA purity
Suitable for downstream applications such as SNP genotyping by PCR or NGS
Free of ethanol, allowing for safer operation and removal of drying times
Ready-to-use kit
Fast and convenient protocols that are easily adaptable to high-throughput automation
Specifications
Compatible with a wide range leaf and seed sample types